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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Acaricidal Effect of Plant Crude-extracts on Tropical Cattle Ticks (Boophilus microplus)) ผู้เขียน: ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์ , ดร.สุรัตน์วดี จิวะจินดา , นายวีระพล จันทร์สวรรค์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe 171 ethanol crude-extracts from 151 plant species were in vitro tested for acaricidal activity on adult tropical cattle ticks by the dipping method. The 10% solution of crude-extract of Calotropis gigantea R. Br. And Streblus asper Lour. Exhibited high acaricidal activity (92% and 100% corrected mortality of ticks 48 h after dipping). Relatively high acaricidal activity (71-85% mortality) was found in the crude-extracts of Aganorcrion polymorphum P.S., Datura metel L. and Rhinacanthus nasutus Kurz. Acaticidal activity of some crude-extracts was observed 5 days after dipping, Which might indicate delayed toxicity. The high delayed acaricidal activity ( 86-100% non laying ) was occurred in Alpinia officinarum Hance, Annona muricata Llk Calotropis procera R.Br., Michelia champaca L;, Pachyrrhisus erosus L., Plumbago zeylandica L, Polyscias balfouriana B., Premna latifolia Roxb., Thunbergia erecta T. Anders., and Thunbergia laurifolia Lindl.. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Acaricidal Effect of Practical Crude-extracts of Plants against Tropical Cattle Ticks ) ผู้เขียน:ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์,  Weerapol Jansawant สื่อสิ่งพิมพ์:pdf Abstract The acaricidal activity of the plant crude-extracts to tropical cattle ticks were studied in the following plants; Calotropis gigantica R.Br., Calotropis procera R.Br., Cr-ytostegia grandiflora R.Br., Homalonlena aromatica Linn., Kalanchoepinnata Lamk., Pachyrrhizus erosus Lion., Polyscias balfouriana Baill, Rauwolfia serpentina B.K.,Rhinacanthus nasutus Linn., Spilanthes acmella Murr., Streblus aspen Lour., and 7hunbergia erecta T. Anders. The crude-extracts of these plants were extracted with 10% ethanol, water, boiling water and boiled water added with the ethanol to 10%. These crude-extracts were tested for the acaricidal activity to the engorged female tropical cattle ticks (Boophilus microplus). Only the crude-extracts of P. erosus with boiling water showed delayed acaricidal activity (anti-laying activity). The crude-extracts obtaining by different times of extraction with boiling water and hot water were stored in refrigerator and tested on both larvae and engorged female ticks with different dilution. The boiling-water and the hot-water crude-extracts for 20 and 40 min. showed high delayed acaricidal activity at 1:6 and 1:5 dilution, respectively. Both of them were stored in refrigerator for 7 days after extraction. The water boiling for 5-40 min. crude-extracts were stored in the refrigerator for 20 days, exhibited relatively high larvicidal activity at 1: 220 dilution. While the hot water extractfor5-40 min. showed high larvicidal activity at 1: 220 dilution after storing in the refrigerator for 12 days. This indicates that acaricidal substances ofP. erosus (yarn bean) seeds can be easily extracted, and the practical application of this crude-extracts to controlling the tropical cattle tick is expectable. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Acaricidal Activity of the Combination of Plant Crude-extracts to Tropical Cattle Ticks) ผู้เขียน:ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, Chainarong Ratanakreetakul, นายวีระพล จันทร์สวรรค์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThirty four ethanol crude-extracts of plant species were studied the acaricidal activity of the two combined crude-extracts to the engorged female tropical cattle tick (Boophilus microplus). The concentration of crude-extract was 2.5% in mixed solution of 9 parts of 1% Tween 80? and 1 part of 100% ethanol. The acaaricidal activity of the combined crude-extracts were tested by dipping method and observed the corrected mortality of the ticks after dipping 24 h, 48 h and 7 days. The acute acaricidal activity were highly found in the combined crude-extracts as follows; Aganonerion polymorphum Pierre ex Spire (Som-lom) with Calotropis procera R.Br. (Rak-dok-khaao), A. polymorphum with Anethum graveolens L.. (Phak-chee-laao); C. procera with Pentapetes Phoenicia L.. (Po-seng). The delayed acaricidal activity were highly wxhibited in the combined crude-extracts of A. polymorphum with Calotropis gigantean R.Br. (Rak-dok-muang), A. polymorphum with Cryptostegia grandiflora R.Br. (Baan-buree-muang), A. polymorphum with lxora nigricans Wight & Arn. (Lum-phuk), C. gigantean with C. grandiflora, C. gigantea with P.phoenicia, I. nigricans with A. graveolens, and Annona muricata L. (Thu-rian-thet) with Piper nigrum L. (Phrik-thai-dam). This results indicated that the practical application of the combination of two crude-extracts of these common plants to control the tropical cattle tick is possible. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Acaricidal Activity of Volatile oil from Lemon and Citronella Grasses on Tropical Cattle Ticks) ผู้เขียน:ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, ดร.สุรัตน์วดี จิวะจินดา สื่อสิ่งพิมพ์:pdf AbstractVolatile oil was obtained from lemon grass (Cymbopogon citratus stapf.) and citronella grass (Cymbopogon nardus Rendle.) leaves by steam distillation. The yields were 0.39% and 0.68% from the fresh leaves of lemon and citronella grasses respectively. Oil yield from dried leaves of lemon and citronella grasses were 0.52% and 1.20%, respectively. The stems and roots gave very less amount of oil. The oil was diluted with 95% ethanol and tested for acaricidal activity on larvae and engorge female cattle ticks (Boophilus microplus) by dipping method. The oil from fresh lemon grass showed high larvicidal activity (1-2 h after dipping) at the concentration of 1:8, 1:12 and 1:16 (oil/ethanol; V/V), while the oil from fresh citronella grass exhibited high activity at concentration of 1:8 and 1:12 (V/V). On the engorge female ticks, the lemon grass oil concentration of 1:2, 1:3 and 1:4 (oil/ethanol; V/V) exhibited high acaricidal activity on 5 days after dipping, but the citronella oil showed high activity at concentration of 1:2 and 1:3. The oil from dried leaves of both grasses showed weaker acaricidal activity on both larvae and adult ticks. In vivo acaricidal bioassay, the ethanol dilution 1:3 (V/V) of both oils were tested by spraying to the tick on cattle. The pre-engorge stage ticks collapsed and died on cattle’s skin while the collected engorge female ticks could not laid and died at 5 days after spraying. This indicates that volatile oil from lemon and citronella grasses can be practically used for controlling cattle ticks. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Isolation of Acaricidal Substances against Tropical Cattle Ticks from Sugar Apple Seeds) ผู้เขียน:ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, Sugunya Mahatheeranont, Chainarong Rattankkreetakul, ดร.สุรัตน์วดี จิวะจินดา, นายวีระพล จันทร์สวรรค์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractIsolation of acaricidal active substances from sugar apple seeds (Annona squamosa L.) against tropical cattle ticks (Boophilus microplus) was performed. Two active compounds were found in ethyl acetate part of ethanol extracted crude. The compound I (R 0.59) was isolated from twice chromatographed fractions of 60-100% ethyl acetate/toluene (F4-6) and 10, 20% ethanol/ethyl acetate (F7-8), and of 10% ethanol/ethyl acetate (FF 7) by preparative TLC (developed with hexane:acetone; 3:2) and preparative HPLC (reverse phase C subscript 18 column, 85% MeOH in water, 210 nm UV detecter). The compound II (R 0.31) was isolated from triple chromatographed of 80% ethyl acetate/toluene (F5), 45-60% acetone/hexane (F A), and of 40-50% ethyl acetate/toluene (FF B) respectively by preparative TLC (developed with hexane:acetone, 3:2). The compound I and II displayed a highest mass in EI mass spectrum at 623 and 432 mass unit, respectively. The fragmentation pattern of compound I are similar to those known substance; squamocin and acetogenin. The protron NMR spectrum of both compounds displayed the similarly pattern of triterpenoid. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Practical Extraction of Sugar Apple Seeds against Tropical Cattle Ticks) ผู้เขียน:ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, ดร.สุรัตน์วดี จิวะจินดา, Chainarong Ratanagreetakul, นายวีระพล จันทร์สวรรค์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe high acaticidal activity of ethanol crude-extract of sugar apple (Annona squamosa L.) seeds on tropical cattle ticks (Boophilus microplus) has been reported in our previous papers. The acaricidal activity on larvae was found in column chromatography fractions of 100% toluene; 40%, 60%, 80% ethyl acetate in toluene, and 100% ethyl acetate. The high acaricidal activity on adult ticks was found in fractions of 40%, 60% ethyl acetate in toluene, 100% ethyl acetate, and 5% ethanol in ethyl acetate. This showed that the active substance was dissolved in polar solvents. Therefore, it might be extracted with water for practical use of the crude extract. For the practical extraction of the active compound from sugar apple seeds, different solvents were examined and tested with regard to effect on adult ticks; water extract, boiling water extract, 10% and 20% ethanol extract, and 11.66% white spirit extract. The 10% ethanol extract and boiling water extract showed high acaricidal activity (87% and 88% mortality of the ticks 48 h after dipping, respectively), even with 2 times dilution by water. The mixture of 10% ethanol extract and it s rinsed solution (1:4 W/V, 33%) still showed activity even after 50 days kept in refrigeration. This indicates that the acaricidal substances of sugar apple seeds are dissolved in polar solvents and simplified extraction for practical use in controlling ticks is possible. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Bioassay Techniques of Insecticidal Plant - Extracts on Tropical Cattle Ticks (Boophilus microplus)) ผู้เขียน:ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, นายวีระพล จันทร์สวรรค์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe engorge female of tropical cattle ticks survived in vitro more than 72h and began laying at 70-75h after taking from cattles. The larvae were hatches 14-23 days after laying. The unfed larvae survived more than 2 months in humid bottle. The leg contact method was used for larvicidal bioassay. The larvae were left to contact with the dry film of plant-extract. The mortality of larvae in empty, evaporated ethanol-and ethyl acetate- coated bottles as control were considerably low and no significant difference were found among them. In insecticidal bioassay of adult ticks, both body dip0ing and leg contact methods could be applied. The dipping method was rapid and easy procedure for screening bioassay. The leg contact methods was used for bioassay in the fractionation of compounds dissolved in non polar organic solvents. The mortality of engorge female ticks was very low at 48 h after dipping with water, methanol, ethanol, acetone and 2% polyoxyethylene sorbitan mono – oleate. After dipping with ethyl acetate and toluene the mortality were high. The survival checks of larvae was observed on its leg movement after exposing to fresh air. The treated engorge female tick was observed in its leg and body movement after exposing to warm-air, 100w lamp’s light sunshine and direct press to the body. The non survival ticks were confirmed by its non laying. This study demonstrated that the cattle ticks can be bioaassayed for larvicidal and insecticidal activities in vitro before further application in vivo |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Light microscopic Identification Type II and Type III Acini Cells
of Salivary Gland of Tropical Cattle Ticks (Boophilus microplus)) ผู้เขียน:ดร.ธรรมาพร พิจิตราศิลป์, ผู้ช่วยศาสตราจารย์ , Worawut Rerkamnuychoke, ดร.ศิริรักษ์ จันทครุ, ผู้ช่วยศาสตราจารย์, Daru Srangam, Somchai Nismeungpuk, Aswin Gingkaew, ดร.สถาพร จิตตปาลพงศ์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractLight microscopic study the cell types in the acinus of salivary gland of tropical cattle tick
(Boophilus microplus). The result showed that six different types of cells can be identified in thicksections
stained with Methylene blue Azure II - Basic fuchsin. The identification of the cells was based
on the difference in staining of their granules as well as their morphology of the cells. In type II acinus
‘a’ cells containing granules which were spherical, packed tight together and stained in deep blue whereas
‘b’ cells had a less granules than ‘c’ cells, their granules appeared in loosely pattern and most of granules
were stained in blue and some in pink. The ‘c’ cells were classified into two types, granular and agranular
cells. The granular ‘c’ cell, contained pink and blue granules. In type III acinus ‘d’ cell had granules which
were similar to those of ‘a’ cells. Granules of ‘e’ cells were larger than those of ‘d’ cell and stained in
blue to light blue and ‘f ‘cell were agranular cells. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Acaricidal Activity of Peel Oil of Citrus spp. On Boophilus microplus) ผู้เขียน:Narong Chungsamarnyary, นายวีระพล จันทร์สวรรค์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractPeel oil from three varieties of Citrus maxima Merr. (pomelo): Khaao-phuaing (immature fruit), Thong-dee (immature and mature fruits) and Khaao-nam-phueng (mature fruits); Citrus reticulata Blanco., Citrus suncris Linn., Citrus sinensis Linn. And Citrus hystrix DC. were collected by mechanical pressing. These peel oil and the d-limonene substance were diluted with 95% ethanol into 1:5, 1:10 and 1:5 (V/V) and tested the acaricidal activity on the engorged female cattle tick (Boophilus microplus) by dipping method. The acaricidal activity on the tick larvae was tested with these oil and d-limonene dilution of 1:10 and 1:15 (V/V). The peel oil of C.reticulata and C.maxima variety thong-dee (immature and mature fruits) showed high acaricidal activity at the concentration of 1:10 (V/V) on engorged female ticks. These peel oil activity were 2 times thigher than that of d-limonene. The peel oil concentration 1:10 of C.sinensis and C.maxima (mature) exhibited high larvicidal activity, while the oil of C.hystrix, C.reticulata, C.suncris and C.maxima (immature fruits) showed relatively high larvicidal activity. Their larvicidal activity were 1.5 times stronger than d-limonene. The peel oil of the other citrus spp. Exhibited weaker acaricidal activity. This indicated that the peel oil from the citrus fruits could be directly applied to control the cattle tick. The waste of the over fruiting immature pomelo, would be of potential usage as a biocontrol substance. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Tamarindus indicus L. Against the Boophilus microplus) ผู้เขียน:ดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, นายวีระพล จันทร์สวรรค์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe crude-extract of Tamarindus indicus L. with water and 10% ethanol in water were tested the acaricidal activity on the engorged female cattle tick (Boophilus microplus) by dipping method. The mature tamarind fruits taking off the seeds were extracted by water or 10% ethanol in the ratio of 1:2 and 1:5 W/V for 7 days. The corrected mortality of the ticks were observed after dipping at 24 h, 48 h and 7 days. The mean of corrected mortality of ticks of these 4 crude-extracts of tamarind fruits were 56-70 %, 70-89% and 77-99% by no statistically significant different after dipping at 24 h, 48 h and 7 days, respectively. The organic acids in tamarind fruits (oxalic, malic, succinic, citric and tartaric acids) were also bioassayed the acaricidal activity by dipping method. The oxalic acid of 0.5% and 1% concentration exhibited the highest acute acaricidal activity (56% and 62% mortality of ticks at 24 h after dipping, respectively). The tartaric acid 1% concentration showed the highest delayed acaricidal activity (73% mortality of ticks at 7 days after dipping). The mixture of 0.5% of oxalic acid with 0.5% of malic, succinic, citric and tartaric acids by concentration of 1:1 V/V were tested the acaricidal activity. The acaricidal activity of these acid mixtures were not more stronger than those of each individual acid. Both of crude-extract of tamarind fruits and their organic acids caused the patchy hemorrhagic swelling on the skin of ticks after dipping at 15 min. This indicates that the crude-extract of tamarind fruits by water or 10% ethanol is possibly use in practical for controlling the tropical cattle tick. The active substances are their organic acids, especially oxalic and tartaric acids. |
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 Researcherดร. ณรงค์ จึงสมานญาติ, รองศาสตราจารย์ที่ทำงาน:สำนักงานเลขานุการ คณะสัตวแพทยศาสตร์ สาขาที่สนใจ:มหกายวิภาคศาสตร์และจุลกายวิภาคศาสตร์ของสัตว์เลี้ยง, การวิภาคศาสตร์ประยุกต์ทางสัตวแพทย์ของม้า สัตว์เคี้ยวเอื้อง สุกร สุนัข แมวและไก่, การทดสอบฤทธิ์ฆ่าเห็บโค สุนัข Resume |
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